HE Promote

BBa_K1539055 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1539055

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Coleen Tran

Date created: 2014-10-09 11:00:00

Date modified: 2015-05-08 01:10:52

HE Promoter Primer

Details

• Types: DnaRegion
• Roles: Primer; primer
• Sequences: BBa_K1539055_sequence (Version 1)

Description

This primer can be used to insert a high efficiency promoter to translate E. coli. genes. Must be used after insertion of RBS primer. Use Georgia Tech iGEM RBS primers (BBa_K1539021 or BBa_K1539034) or an RBS primer with a similar design.

Notes

Primer design could not retain the EcoRI site but once the construct (promoter->RBS->gene) is reinserted into pSB1C3 backbone, EcoRI is site is reestablished.

Source

Primers ordered from IDT

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1539055/1

Member of these Collections

• iGEM Parts Registry