Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marc Slingerland, Nathan Puhl
Date created: 2008-10-24 11:00:00
Date modified: 2015-05-08 01:10:53
LIC plasmid
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K155000_sequence (Version 1) |
Description
This is a plasmid intended for use in ligation-independent cloning. The overhang sequence is based on Aslanidis & DeJong (1990) "Ligation-independent cloning of PCR products (LIC-PCR)." Nucl. Acids Res, 18:20, 6069-6074.Notes
Added an EcoRV restriction site; had to be compatible with the NTP requirements of the overhangs.Source
From PCR amplification of pSB1A2 with overhange primers.| Sequence Annotation | Location | Component / Role(s) |
| EcoRV site EcoRV site LIC overhang VR rep (pMB1) (rev) ORI APr (rev) pBLA (rev) VF2 LIC overhang (rev) terminator | 1,3 2066,2068 1,15 74,93 177,792 193,193 951,1812 1847,1847 1935,1954 2053,2068 2024,2043 | sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/primer_binding primer_binding_site feature/cds CDS feature/conserved conserved_region feature/cds CDS feature/conserved conserved_region primer_binding_site feature/primer_binding sequence_feature feature/misc stem_loop feature/stem_loop |