| Types | DnaRegion
|
| Roles | engineered_region
Composite
|
| Sequences | BBa_K1550000_sequence (Version 1)
|
Description
This part includes the strong RBS BBa_B0034 (Elowitz, 1999), BmtA from Oscillatoria brevis, which encodes lead binding metallothionein (Liu et al., 2003), and the double transcription terminator BBa_B0015. The BmtA sequence in this part has been codon optimized for E. coli. This part can be used with a constitutive or inducible promoter to produce metallothionein in projects utilizing the metal-binding protein for sequestration purposes.
Notes
When used in E. coli for the sequestration of lead or zinc ions in solution, the part may or may not work better in concert with a genomic ZntA knockout. We plan to characterize this combination in the near future.
Source
The sequences for pre-existing basic parts (BBa_B0034 and BBa_B0015) were incorporated as found in the iGEM registry. The sequence used for the BmtA (metallothionein) was codon optimized for E. coli (using the browser-based software, Gene Design). The native sequence comes from Oscillatoria brevis, and was sourced from an entry in the NCBI database by Liu,T., Nakashima,S., Hirose,K., Uemura,Y., Shibasaka,M., Katsuhara,M. and Kasamo,K. (2003).
