BBa_K1554005

BBa_K1554005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1554005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Valencia_UPV team members
Date created: 2014-09-28 11:00:00
Date modified: 2015-06-09 09:45:18

Blue biosafety module for plants



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K1554005_sequence (Version 1)

Description

This biosafety device for plants consists on two submodules: a male sterility submodule and an identity preservation submodule. The male sterility submodule consist on a barnase specifically expressed in anthers under the regulation of the TA29 tapetum-specific promoter. As result, pollen from these plants is not fertile. The identity preservation submodule expresses the blue chromoprotein AmilCP, so that plants can be visually differentiated from non-transgenic plants.

Notes

The assembly was performed by GoldenBraid 2.0 assembly. The barnase was amplified by PCR from Part:BBa_J72173 and a mutation in T105P was introduced.

Source

TA29 promoter Part:BBa_K1554000

Barnase Part:BBa_J72173

AmilGFP [Waiting for Norwich team part name in registry]

P35s Part:BBa_K1537015

Sequence Annotation Location Component / Role(s)
TA29 promoter
barnase
T35s
P35s
AmilCP
T35S
4,858
863,1195
1200,1425
1438,2464
2469,3131
3136,3361
feature/promoter promoter
CDS feature/cds
feature/stop stop_codon
feature/promoter promoter
CDS feature/cds
stop_codon feature/stop
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1554005/1