BBa_K156001

BBa_K156001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K156001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: George McArthur IV

Date created: 2008-10-27 12:00:00

Date modified: 2015-05-08 01:10:54

BioBrick Placeholder 1 (Prefix - ApoI (NotI) AvrII - Suffix)

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_K156001_sequence (Version 1)

Description

This BioBrick Placeholder part is the first of eight and represents a new technical standard useful in composite part construction. These parts are made of restriction sites that are compatible with the standard BBa sites (see below) and serve as placeholders for future inserts (e.g., RBSs, coding regions). This allows insertion of a desired part within a previously-made construct.

Compatible sites:

EcoRI - ApoI & MfeI
X & S - AvrII, NheI
PstI - NsiI, SbfI

Notes

Sites are compatible with standard BBa restriction sites and will form scars when ligated.

Source

The DNA sequence is simply linked restriction enzyme sites that are compatible with standard BBa restriction sites. These compatible sites were compiled and listed in "Engineering BioBrick vectors from BioBrick parts". http://www.jbioleng.org/content/2/1/5

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K156001/1

Member of these Collections

• iGEM Parts Registry