Bioplastic

BBa_K156012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K156012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: George McArthur IV
Date created: 2008-10-28 12:00:00
Date modified: 2015-05-08 01:10:54

phaA (acetyl-CoA acetyltransferase)



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K156012_sequence (Version 1)

Description

This part codes for an enzyme that catalyzes the synthesis of acetoacetyl-coenzyme A from two molecules of acetyl-coenzyme A. The enzyme can also act as a thiolase, catalyzing the reverse reaction and generating two-carbon units from the four-carbon product of fatty acid oxidation.

Notes

This part was codon-optimized for expression in E. coli. The following restriction enzyme sites were also removed: EcoRI, NotI, XbaI, SpeI, PstI, NheI, AvrII, ApoI, MfeI, NsiI, SbfI.

Source

This coding region is native to Ralstonia eutropha H16. The original sequence was obtained from GenBank (VERSION NC_008313.1, GI:113866031).

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
rdf-schema#seeAlso
P14611
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K156012/1