Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Deborah M Landim Zanforlin, Roberta Godone, Mirella Monteiro
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:54
ColiAlert
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1565002_sequence (Version 1) |
Description
This part is responsible for the pink coloration of E. coli. E. coli transformed with this biobrick becomes pink in neutral and basic medium (LB culture medium for example). However, cultures can change color to yellow (instantly) with the acidification of the medium. Another factor that makes the medium yellow, is the addition of cell lysis buffer. The cells lysed immediately becomes yellow, while those that are not lysed remain pink. Thus, this biobrick can be used as an indicator of cell lysis.Notes
The design was thought to expression of yellow coloration. However, in basic medium, there expressing pink color.Source
This biobrick is formed by other biobricks the registry.| Access | Instance | Definition |
| public public public public | BBa_I746361 BBa_K554002 BBa_K1033910 BBa_B0015 | BBa_I746361 BBa_K554002 fwYellow BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I746361 BBa_K554002 BBa_K1033910 BBa_B0015 | 1,92 101,286 293,1006 1015,1143 | BBa_I746361 BBa_K554002 fwYellow BBa_B0015 |