Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Team Freiburg 2008
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:10:54
Split-Cerulean-cCFP
| Types | DnaRegion |
| Roles | polypeptide_domain Protein_Domain |
| Sequences | BBa_K157005_sequence (Version 1) |
Description
C-terminal fragment (Amino acids156-236) of the monomeric[5] cyan fluorescent protein variant ???Cerulean???, designed for fusion/BiFC[1,3]. Reassembly with Split-Cerulean-nCFP (Part Bba_K157006) generates the complete, working CFP ???Cerulean???, whereas combination with the N-terminal fragment (Part Bba_K157008) of the monomeric yellow fluorescent Protein ???Venus???[4] (Part Bba_I757008) results in green fluorescence[2].References see "Part Design".Notes
Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.Source
Gene synthesis by GeneArt, optimized for expression in homo sapiens.| Sequence Annotation | Location | Component / Role(s) |
| C-Terminal portion of cerulean CFP | 1,243 | CDS feature/protein |