Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Chenmao Wang
Date created: 2015-08-05 11:00:00
Date modified: 2015-09-16 08:09:09
GFP+sJanus Fusion Protein
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1582004_sequence (Version 1) |
Description
HFBI is a modle of class I of hydrophobins, which are naturally produced by filamentous fungi. Hydrophobins are amphipathic, which means they have the hydrophobic part and hydropholic part at the same time. HFBI is a kind of hydrophobins and it has magic properties. We combine GFP with HFBI by a linker and make them become a fusion protein. This fusion protein could be used in aqueous two-phase systems to make itself seperated frome mixture. Meanwile, hydrophobin has a property that it can generate a membrane on the surface of solid automatically, which is called self-assembly. We can get a fluorescent protein' membrane by it.Notes
When amplify the HFBI and GFP with PCR, temperature is important.Source
GFP comes frome iGEM's standard part and HFBI comes frome Trichoderma reesei.| Access | Instance | Definition |
| public public | BBa_K1582032 BBa_K1582034 | BBa_K1582032 BBa_K1582034 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1582032 BBa_K1582034 | 1,714 715,1017 | BBa_K1582032 BBa_K1582034 |