Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Max van 't Hof
Date created: 2015-09-03 11:00:00
Date modified: 2015-09-04 04:16:37
CsgA_His
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1583003_sequence (Version 1) |
Description
This gene encodes for the protein CsgA with a HIS-tag attached to it. CsgA is a curlin subunit that can self-assemble into curli after nucleation on CsgB. They are produced in an unpolymerized form and secreted from the cell.CsgA is naturally produced by e.g. E. coli K-12 MG1655 from the operon CsgBA. A second operon, CsgDEFG encodes four additional proteins that are required for the curli assembly and secretion.
Notes
The nucleotides encoding for the third and fourth amino acids of the gene are changed to improve the success change of synthesisSource
The DNA was synthesized. The sequence of CsgA originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055). The design was based on the paper "Programmable biofilm-based materials fromengineered curli nanofibres"[1]
1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.