Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Max van 't Hof
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-14 01:44:54
SpyCatcher without startcodon + His-tag
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1583018_sequence (Version 1) |
Description
SpyCatcher is a protein that can spontaneously form covalent isopeptide linkages with it??s counterpart `Spytag` under physiological conditions.Notes
Startcodon was not included, since BFP (BBa_K1583017) was placed in front of this biobrick.Source
This gene was synthesized as part of biobrick BBa_K1583113. The idea of using Spytag/SpyCatcher was based on the paper "Programmable biofilm-based materials fromengineered curli nanofibres"[1]1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.
| Sequence Annotation | Location | Component / Role(s) |
| SpyCatcher His-tag Stop-codon | 1,387 388,405 406,408 | feature/protein CDS non_covalent_binding_site feature/binding stop_codon feature/stop |