Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Max van 't Hof
Date created: 2015-09-02 11:00:00
Date modified: 2015-09-18 11:09:29
pRha + CsgA
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1583100_sequence (Version 1) |
Description
CsgA under control of L-rhamnose-inducible promoterNotes
The nucleotides encoding for the second and third amino acid of the CsgA gene were changed to optimize synthesis success. (silent mutations)Source
This part was synthesized. The rhamnose promoter was used by the iGEM 2014 TU Delft teama and originally added by the iGEM12 Paris Bettencourt team. RBS and CsgA originate from E. coli K-12 MG1655| Access | Instance | Definition |
| public public public public public public | BBa_G0002 BBa_K914003 BBa_K1583050 BBa_K1583008 BBa_K1583000 BBa_K1583051 | SX scar BBa_K914003 BBa_K1583050 BBa_K1583008 BBa_K1583000 BBa_K1583051 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_G0002 BBa_K914003 BBa_K1583050 BBa_K1583008 BBa_K1583000 BBa_K1583051 | 1,8 9,130 131,155 156,167 169,624 625,638 | SX scar BBa_K914003 BBa_K1583050 BBa_K1583008 BBa_K1583000 BBa_K1583051 |