BBa_K1583200

BBa_K1583200 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1583200
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Robert Marsden
Date created: 2015-09-03 11:00:00
Date modified: 2016-01-25 11:28:10

Low constitutive promoter + CsgC



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1583200_sequence (Version 1)

Description

CsgC is positively involved in the extracellular aggregation of CsgA into amyloid nanowires (curli assembly) in E.coli functioning as a chaperone for CsgA. Constructs with different promoter strenghts were designed to investigate the impact on nanowire self-assembly.

Notes

We inserted non-coding scar sequences after the promoter and RBS to prevent missing initial codons during transcription/translation.

Source

The RBS and CsgC were synthesized from the genomic sequence of E.coli K-12 MG1655.
The promoter is part of the constitutive promoter family made by John Anderson from iGEM Berkeley 2006.
Non-coding scar sites were designed at random.

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1583200/1