Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Robert Marsden
Date created: 2015-09-03 11:00:00
Date modified: 2016-01-25 11:28:24
Medium constitutive promoter + CsgC
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1583201_sequence (Version 1) |
Description
CsgC is positively involved in the extracellular aggregation of CsgA into amyloid nanowires (curli assembly) in E.coli functioning as a chaperone for CsgA. Constructs with different promoter strenghts were designed to investigate the impact on nanowire self-assembly.Notes
We inserted non-coding scar sequences after the promoter and RBS to prevent missing initial codons during transcription/translation.Source
The RBS and CsgC were synthesized from the genomic sequence of E.coli K-12 MG1655.The promoter is part of the constitutive promoter family made by John Anderson from iGEM Berkeley 2006.Non-coding scar sites were designed at random.
| Access | Instance | Definition |
| public public public public public | BBa_J23118 BBa_K1583053 BBa_B0034 BBa_K1088022 BBa_K1583001 | BBa_J23118 BBa_K1583053 BBa_B0034 BBa_K1088022 BBa_K1583001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23118 BBa_K1583053 BBa_B0034 BBa_K1088022 BBa_K1583001 | 1,35 36,53 54,65 66,71 72,404 | BBa_J23118 BBa_K1583053 BBa_B0034 BBa_K1088022 BBa_K1583001 |