Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nu Ri Choi, Seung Meen Choi, SOOJI LEE, Brittany LEE, Hyun Min Park
Date created: 2015-09-12 11:00:00
Date modified: 2015-09-17 07:51:52
LuxR-responsive promoter with LacZ CDS
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1584036_sequence (Version 1) |
Description
This part contains two CDSs for expression in bacteria. The first is LuxR which is controlled by a constitutive left and a right promoter. The second is LacZ which is controlled by promoter with a LuxR recognition site. LuxR protein forms a complex with quorum sensing signalling molecule homoserine lactone,which then increases the rate of transcription. Therefore,in the presence of homoserine lactone, the LacZ reporter is expressed.Notes
J61100 has two XbaI sites, so we cut J61100 with XbaI.Source
We used promoter(R0062), RBS(J61100) and lacZ(I732006).| Access | Instance | Definition |
| public public public public | BBa_K823017 BBa_R0062 BBa_J61100 BBa_I732006 | BBa_K823017 lux pR BBa_J61100 lacZ-alpha |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K823017 BBa_R0062 BBa_J61100 BBa_I732006 | 1,95 104,158 167,178 185,418 | BBa_K823017 lux pR BBa_J61100 lacZ-alpha |