Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alexandre Le Scornet
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-16 11:58:25
Butyrate synthesis pathway regulated by constitutive promoter p(Bla)
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1587004_sequence (Version 1) |
Description
Constitutive promoter p(Bla). Crotonyl-coA reductase ccr coding region. 3-hydroxybutyryl-CoA dehydrogenase hbd coding region. 3-hydroxybutyryl-CoA dehydratase crt coding region. Acyl-CoA thioesterase 2 tesB coding region. acetyl-CoA acetyltransferase atoB coding region. Every genes are framed by strong RBS based on Ron Weiss thesis. This operon ends with an artificial terminator according to BBa_B1006.Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol. Unfotunatly NMR analysis did not yield a significant over production of butyrate in E. coli.
Notes
p(Bla) is followed by NheI restriction site and ccr is followed by XhoI restriction siteSource
tesB, ccr, crt, and hbd sequence are from Clostridium acetobutylicum. atoB sequence is from E.coli. The strong RBS and Terminator were used according to respectively BBa_B0030 and BBa_B1006| Sequence Annotation | Location | Component / Role(s) |
| p(Bla) rbs ccr rbs hbd rbs crt rbs tesB rbs atoB Terminator | 1,35 42,56 57,1406 1413,1427 1428,2276 2277,2291 2292,3077 3078,3092 3093,3953 3954,3968 3969,5153 5154,5192 | promoter feature/promoter feature/rbs ribosome_entry_site feature/protein CDS ribosome_entry_site feature/rbs feature/protein CDS ribosome_entry_site feature/rbs CDS feature/protein ribosome_entry_site feature/rbs feature/protein CDS feature/rbs ribosome_entry_site feature/protein CDS sequence_feature feature/misc |