Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Louise GODY
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-16 10:53:43
Butyrate synthesis pathway (BBa_1587004) without ccr gene regulated by constitutive promoter p(Bla)
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K1587005_sequence (Version 1) |
Description
Constitutive promoter p(Bla). 3-hydroxybutyryl-CoA dehydrogenase hbd coding region. 3-hydroxybutyryl-CoA dehydratase crt coding region. Acyl-CoA thioesterase 2 tesB coding region. acetyl-CoA acetyltransferase atoB coding region. Every genes are framed by strong RBS based on Ron Weiss thesis. This operon ends with an artificial terminator according to BBa_B1006.Due to a lack of time we decided to have this construction synthesised instead of using the assembly protocol.
This construction without the ccr gene is a negative control of the butyrate production in E. coli. Moreover, it was aimed to be inserted in our regulation system.
This construction may be used in order to produce all the intermediates of butyrate up to crotonyl-CoA.
Notes
No informationSource
We did the assembly of several sequences from ncbi, and hbd and crt genes which came originally from Clostridium acetobutylicum were optimized to work in E.coli.| Sequence Annotation | Location | Component / Role(s) |
| p(Bla) rbs hbd rbs rbs crt tesB rbs atoB Terminator | 1,35 48,62 63,911 912,926 1713,1727 927,1712 1728,2588 2589,2603 2604,3788 3789,3827 | promoter feature/promoter feature/rbs ribosome_entry_site CDS feature/protein ribosome_entry_site feature/rbs feature/rbs ribosome_entry_site feature/protein CDS feature/protein CDS ribosome_entry_site feature/rbs feature/protein CDS feature/misc sequence_feature |