BBa_K1590003

BBa_K1590003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1590003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Manuel Blank
Date created: 2015-09-08 11:00:00
Date modified: 2015-09-18 03:49:07

Chromate responsive promoter



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_K1590003_sequence (Version 1)

    Description

    The promoter pChr is suspected to be inducible by chromate.

    Notes

    The promoter was excised as a separate part in order to build a chromate-inducible fluorescent reporter.

    Primers for amplification of pChr from BBa_K1058008.

    Forward: GCGC GAATTCGCGGCCGCTTCTAGAG ATTGCTTATTCCTATTGC
    Reverse: GCGC CTGCAGCGGCCGCTACTAGT AGTCGTAGATGTTACTACA

    Source

    PCR-amplification from biobrick BBa_K1058008 (pChrBGFP).

    Sequence Annotation Location Component / Role(s)
    putative repressor binding site
    		133,165
    		feature/binding non_covalent_binding_site

    igem#experience
    Works
     
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    Planning
     
    synbiohub#ownedBy
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    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1590003/1
     

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