Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Manuel Blank
Date created: 2015-09-08 11:00:00
Date modified: 2015-09-18 03:51:13
Regulator or Chromate responsive promoter
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1590004_sequence (Version 1) |
Description
The protein encoded by this sequence is a putative chromate responsive repressor of pChr (BBa_K1590003).Notes
The part was partially optimised by altering the first 15 codons by means of a non-specific primer. The codons were biased towards maximum expression level in E. coli K12 strains.Primers used for amplification of ChrB coding-region, while optimising the 15 codons, adding a standard prefix, and a standard suffix to it:
Forward: GCGC GAATTCGCGGCCGCTTCTAG A TGAACCTGCTGTCTCCGATCCTGTCTCTGCCGACCGAAAACGCG
Reverse: GCGC CTGCAGCGGCCGCTACTAGT ATTATT ATACGGTGAGGGTCCC
Primers for amplification of partially optimised ChrB from BBa_K1058008 and adding a standard prefix, a PstI restriction site, and a His6-tag to it.
Forward: GCGC GAATTCGCGGCCGCTTCTAG A TGAACCTGCTGTCTCCGATCCTGTCTCTGCCGACCGAAAACGCG
Reverse: GCGC CTGCAG TTATTA ATGGTGATGGTGATGGTGTACGGTGAGGGTCCC
Primers for amplification of partially optimised ChrB from recombinant plasmid and adding BamHI/PstI restriction sites and a His6-tag to it.
Forward: GCGC GGATCC ATGAACCTGCTGTCTCCGATCCTGTCT
Reverse: GCGC CTGCAG TTATTA ATGGTGATGGTGATGGTG TACGGTGAGGGTCCC
Source
PCR-amplification from biobrick BBa_K1058008 (pChrBGFP).| Sequence Annotation | Location | Component / Role(s) |
| ATG start codon ChrB coding sequence TAA stop codon optimised for K12 | 1,3 1,936 937,939 1,15 | feature/start start_codon feature/cds CDS feature/stop stop_codon sequence_feature feature/misc |