Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Manuel Blank
Date created: 2015-09-09 11:00:00
Date modified: 2015-09-18 04:06:15
Human Lanosterol Synthase
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1590006_sequence (Version 1) |
Description
Lanosterol Synthase catalyses the reaction from 2,3-oxido-squalene (squalene epoxide) to Lanosterol. It is one of the enzymes in the enzymatic cascade that converts squalene to cholesterol through stepwise modifications of the substrate.Notes
Choosing the correct codon-bias, remove 'illegal' restriction sites, ensure that (stop)codons are in frame, addition of standard prefix and suffix.Intermediate primers for sequencing Lanosterol Synthase.
Forward: GGCTGTTCCCGGACTGGG
Reverse: CGGTGACATCATGATCGAC
Primers for cloning Lanosterol Synthase into overexpression vector pQE80-L.
Forward: GCGCGGATCCATGACCGAAGGAACCTGC
Reverse: GCGCGGTACCTTACGGGTGACCCGCCAGCGC
Part was synthesised with appropriate prefix/suffix sequences, hence no separate primers were required for cloning it into pSB1C3.
Source
In silico optimised version for E. coli K12 strains. Sequence ordered through IDT.| Sequence Annotation | Location | Component / Role(s) |
| ATG start codon Human Lanosterol Synthase Protein Coding Sequence TAA stop codon | 1,3 1,2196 2197,2199 | start_codon feature/start feature/cds CDS stop_codon feature/stop |