Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Manuel Blank
Date created: 2015-09-08 11:00:00
Date modified: 2015-09-18 04:08:31
Lactoferrin Binding Protein
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1590008_sequence (Version 1) |
Description
Coding sequence for Lactoferrin Binding Protein of Neisseria Meningitidis. This protein sequesters Iron for the host from Lactoferrin.Notes
Biobrick Prefix, Suffix, and TAA stop codons had to be added. Sequence was checked for forbidden restriction sites in order to comply with assembly standard RFC10.Forward primer for amplifying lactoferrin binding protein A from Neisseria meningitidis MC58 gDNA.
Forward: GCGC GAATTCGCGGCCGCTTCTAG A TGAATAAGAAACACGGTTTTCCG
Reverse: GCGC CTGCAGCGGCCGCTACTAGT ATTATT AAAACTTCATTTCGAGCG
Intermediate sequencing primers for Lactoferrin Binding Protein.
Forward 1: GAAGTCGCTCGGCGCGGGG
Forward 2: GACAAGAGCGGGGTGTACG
Reverse : GCCGAATCAGGCAGGAC
Primers for cloning Lactoferrin Binding Protein into overexpression vector pQE80-L.
Forward: GCGC GGATCC AATAAGAAACACGGTTTTCC
Reverse: GCGC GGTACC TTAAAACTTCATTTCGAGCG
Source
PCR amplification of Neisseria Meningitidis MC58 gDNA. gDNA provided by James Moir - University of York.| Sequence Annotation | Location | Component / Role(s) |
| ATG start codon Lactoferrin Binding Protein A Coding Sequence TAA stop codon | 1,3 1,2829 2830,2832 | start_codon feature/start CDS feature/cds stop_codon feature/stop |