Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Manuel Blank
Date created: 2015-09-08 11:00:00
Date modified: 2015-09-18 04:09:09
Spermidine/putrescine-binding periplasmic protein
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1590009_sequence (Version 1) |
Description
Escherichia coli PotD sequence, encoding Spermidine/putrescine-binding periplasmic protein.Notes
Adding biobrick prefix, suffix, and double TAA stop codon to it via primers. Checked for forbidden restriction sites.Primers for amplification of PotD from E. coli MG1655 gDNA and subsequent insertion into pSB1C3.
Forward: GCGC GAATTCGCGGCCGCTTCTAG A TGAAAAAATGGTCACGCCAC
Reverse: GCGC CTGCAGCGGCCGCTACTAGT ATTATT AACGTCCTGCTTTCAGCTT
Primers for cloning PotD into overexpression vector pQE80-L.
Forward: GCGC AGATCTAAAAAATGGTCACGCCAC
Reverse: GCGC GGTACCTTAACGTCCTGCTTTCAGCTTC
Source
PCR amplification of Escherichia Coli MG1655 gDNA.| Sequence Annotation | Location | Component / Role(s) |
| ATG start codon PotD Protein Coding Sequence TAA stop codon | 1,3 1,1044 1045,1047 | feature/start start_codon feature/cds CDS feature/stop stop_codon |