Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Manuel Blank
Date created: 2015-09-08 11:00:00
Date modified: 2015-09-18 04:13:23
Murine Spermine Binding Protein
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1590010_sequence (Version 1) |
Description
Binds the carbohydrate spermine.Notes
Choosing the correct codon-bias, remove 'illegal' restriction sites, ensure that (stop)codons are in frame, addition of standard prefix and suffix.Primers for cloning Spermine Binding Protein into overexpression vector pQE80-L.
Forward: GCGC GGATCC CTGCTGCTGCTGACCCTGGCG
Reverse: GCGC GGTACC TTAAGATTCGTCCTTCTGATC
Part was synthesised with appropriate prefix/suffix sequences, hence no separate primers were required for cloning it into pSB1C3.
Source
In silico optimised version for E. coli K12 strains. Sequence ordered through IDT.| Sequence Annotation | Location | Component / Role(s) |
| ATG start codon Murine Spermine Binding Protein Protein Coding Sequence TAA stop codon | 1,3 1,597 598,600 | start_codon feature/start CDS feature/cds feature/stop stop_codon |