Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuyan Tang
Date created: 2015-09-05 11:00:00
Date modified: 2015-09-18 10:05:23
Mcfp3 with LIP2 prepro
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1592003_sequence (Version 1) |
Description
Mcfp-3 is foot protein secreted from Mytilus californianus. The protein is of significance to the formation of byssus to help mussels permanently or temporarily tether to the surface of solid surface of reef or ship-body. This coherent substance shows the excellent adhesion performance with no substitute under water for sustaining the repeating wash of waves and having no toxicity.The signal tag, LIP2 prepro(BBa_K1592000), fused to Mcfp3 can lead the co-translational translocation of heterologous protein Mcfp-3 to be secreted out of the cell to achieve its function, during the progress of which the peptide will be deleted in the Golgi apparatus to eliminate the interference to the secreting protein. And hexahis-tag added between LIP2 prepro and Mcfp3 can therefore be used for protein purification.
Notes
noneSource
Synthesized by IDT,| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1592000 LIP2 prepro BamHI his-tag Mcfp3 BBa_K1592001 | 1,99 1,99 100,105 106,123 124,357 124,357 | engineered_region feature/BioBrick CDS feature/protein sequence_feature feature/misc feature/tag tag CDS feature/protein engineered_region feature/BioBrick |