BBa_K1592023

BBa_K1592023 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1592023
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuyan Tang
Date created: 2015-09-08 11:00:00
Date modified: 2015-09-09 08:37:50

ptrp mutant1+RBS+GFP



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1592023_sequence (Version 1)

Description

ptrp is a promoter with the trp operator. It will be repressed by trpR (K588000) and LovTAP.
Note that the original trp operator contains two SpeI sites。
We remove the illegal sites without affecting its function by site-directed mutagenesis for meeting the requirements of RFC10 and more convenient to be assembled, and finally constructed three ptrp muant, called ptrp mutant1, ptrp mutant2, and ptrp mutant3.
In order to test the mutants, we construct the RBS and GFP after them.

Notes

none

Source

none

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1592023/1