Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuyan Tang
Date created: 2015-09-08 11:00:00
Date modified: 2015-09-09 08:37:50
ptrp mutant1+RBS+GFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1592023_sequence (Version 1) |
Description
ptrp is a promoter with the trp operator. It will be repressed by trpR (K588000) and LovTAP.Note that the original trp operator contains two SpeI sites。
We remove the illegal sites without affecting its function by site-directed mutagenesis for meeting the requirements of RFC10 and more convenient to be assembled, and finally constructed three ptrp muant, called ptrp mutant1, ptrp mutant2, and ptrp mutant3.
In order to test the mutants, we construct the RBS and GFP after them.
Notes
noneSource
none| Access | Instance | Definition |
| public public public | BBa_K1592020 BBa_B0032 BBa_E0040 | BBa_K1592020 BBa_B0032 GFP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1592020 BBa_B0032 BBa_E0040 | 1,85 94,106 113,832 | BBa_K1592020 BBa_B0032 GFP |