BBa_K1597000

BBa_K1597000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1597000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marieke Mulder
Date created: 2015-09-08 11:00:00
Date modified: 2015-09-13 02:51:51

Salt inducible promoter for B. subtilis



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K1597000_sequence (Version 1)

Description

Bacillus subtilis is capable to cope with fluctuating salt concentrations. One of the genes involved is the proH, this is an 1-pyrroline-5-caboxylate reductase. A studie has shown that this gene is under control of the proH-promoter wich can be activated by a range of salt concentrations.
We have cloned this promoter out of the genome of bacillus and combined it with TasA (an amyloid producing gene) and GFP. The latter was used to validate the promoter.

Notes

During the design primers were designed to add a prefix and suffix to the promoter.

Source

Bacillus subtilius genomic DNA

igem#experience
None
 
igem#sampleStatus
No part sequence
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1597000/1