Types | DnaRegion
|
Roles | Regulatory
promoter
|
Sequences | BBa_K1597000_sequence (Version 1)
|
Description
Bacillus subtilis is capable to cope with fluctuating salt concentrations. One of the genes involved is the proH, this is an 1-pyrroline-5-caboxylate reductase. A studie has shown that this gene is under control of the proH-promoter wich can be activated by a range of salt concentrations.
We have cloned this promoter out of the genome of bacillus and combined it with TasA (an amyloid producing gene) and GFP. The latter was used to validate the promoter.
Notes
During the design primers were designed to add a prefix and suffix to the promoter.
Source
Bacillus subtilius genomic DNA