BBa_K1597000

BBa_K1597000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1597000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Marieke Mulder

Date created: 2015-09-08 11:00:00

Date modified: 2015-09-13 02:51:51

Salt inducible promoter for B. subtilis

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1597000_sequence (Version 1)

Description

Bacillus subtilis is capable to cope with fluctuating salt concentrations. One of the genes involved is the proH, this is an 1-pyrroline-5-caboxylate reductase. A studie has shown that this gene is under control of the proH-promoter wich can be activated by a range of salt concentrations.
We have cloned this promoter out of the genome of bacillus and combined it with TasA (an amyloid producing gene) and GFP. The latter was used to validate the promoter.

Notes

During the design primers were designed to add a prefix and suffix to the promoter.

Source

Bacillus subtilius genomic DNA

Other Properties

• igem#experience: None
• igem#sampleStatus: No part sequence
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1597000/1

Member of these Collections

• iGEM Parts Registry