Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marieke Mulder
Date created: 2015-09-13 11:00:00
Date modified: 2015-09-14 06:42:56
bslA, forms hydrophobic surface layer on biofilm
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1597003_sequence (Version 1) |
Description
To make our biofilm more robust and hydrophobic we we have focussed on bslA (K-nummer). bslA forms a hydrophobic surface layer on the biofilm of Bacillus subtilis. This layer is essential for the water repelling properties of the biofilm of the Bacillus subtilis.The BslA protein itself is a cell wall protein with amphiphilic properties which self-assembles into polymers at the air-water interface in vitro. For the expression of bslA, expression of epsABCDEFGHIJKLMNO and tapA-sipW-tasA operons are required. Without bslA the microstructure of the biofilm is smoothened and the repellency against water and organic solvents is reduced.
Notes
We had to change nucleotide 387 from a cytosine into a thymine in order to get rid of an EcoRI restriction site but not change the amino acid sequenceSource
Genomic DNA from Bacillus subtilis 168| Sequence Annotation | Location | Component / Role(s) |
| bslA original RBS from blsA | 18,563 1,17 | feature/cds CDS ribosome_entry_site feature/rbs |