Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonas Sindlinger, Leon Kraus, Jonathan Pletzer-Zelgert, Elena Tonchevska, Sebastian Holzner, Mirjam Haun, Sebastian Barthel
Date created: 2015-08-27 11:00:00
Date modified: 2015-09-25 01:14:47
B0034-xylB
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K1602000_sequence (Version 1) |
Description
Itaconic acid is an organic, dicarboxylic acid that is biotechnologically synthesized in Aspergillus terreus. It is derived from citric acid via 2 intermediates and a final decarboxylation.
To enable this pathway in Escherichia coli it is necessary to introduce 3 genes. 2 of which are already established in the citric acid cycle and one that is from Apergillus terreus, a cis-aconitate decarboxylase (cadA).
Figure 1 Reaction scheme of the itaconic acid producing operon. The substrates for the reaction are acetyl-coa and oxaloacetate. To simplify the process we are only looking at oxaloacetate right now. Oxaloacetate is metabolized to itaconic acid in 3 steps.
Usage
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- citrate synthase - gltA (BBa_K1033001)
- aconitate hydratase 1 - acnA (BBa_K1033000)
- cis-aconitate decarboxylase - cadA (BBa_K1497100)
iGEM TU Darmstadt 2014 :)
Figure 2
Genetic map of the itaconic acid producing operon with T7 promoter. This brick directs the flux towards and finally enables E.Coli BL21 cells to synthesize itaconic acid in presence of the inductor IPTG.
Notes
BLANKSource
GenBank| Access | Instance | Definition |
| public public | BBa_B0034 BBa_K1602009 | BBa_B0034 BBa_K1602009 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0034 BBa_K1602009 | 1,12 19,765 | BBa_B0034 BBa_K1602009 |