Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Hellgren
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 08:39:44
Fusion GPCR STE2MAM2
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1603000_sequence (Version 1) |
Description
Fusion GPCR of the non-cytoplasmic N-terminal signal peptide from STE2 (Saccharomyces cerevisiae) and Pheromone P-factor receptor MAM2 (schizosaccharomyces pombe)without its signaling peptide. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway.Notes
20 base pair overlapping sequence between the signal peptide from STE2 and MAM2 had to be constructed through PCR to allow Gibson assembly of the two gene fragments. Added Prefix and Suffix through PCR.Source
The non-cytoplasmic N-terminal signal peptide from STE2 is a genomic sequence from Saccharomyces cerevisiae CEN.PK2 and the Pheromone P-factor receptor MAM2, without its signaling peptide, is a genomic sequence from Schizosaccharomyces pombe Δ8 h-.| Sequence Annotation | Location | Component / Role(s) |
| Signal peptide from STE2 MAM2 without signal peptide | 1,153 154,1053 | feature/misc sequence_feature feature/misc sequence_feature |