| Types | DnaRegion
|
| Roles | promoter
Regulatory
|
| Sequences | BBa_K1603001_sequence (Version 1)
|
Description
The high expression pTEF1 promoter connected to pSUC2 promoter. Can be used for induced high expression of any coding part in Saccharomyces cerevisiae at low ATP levels.
Notes
Added 20 overlapping base pairs between the two gene fragments through PCR with primers with overhangs. This allowed the two promoters to be combined through Gibson assembly. Added Prefix and Suffix through PCR with primers with overhangs.
Source
Genomic sequence from Saccharomyces cerevisiae CEN.PK2
