BBa_K1603001

BBa_K1603001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1603001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: John Hellgren

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-18 05:57:31

pTEF1-pSUC2

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1603001_sequence (Version 1)

Description

The high expression pTEF1 promoter connected to pSUC2 promoter. Can be used for induced high expression of any coding part in Saccharomyces cerevisiae at low ATP levels.

Notes

Added 20 overlapping base pairs between the two gene fragments through PCR with primers with overhangs. This allowed the two promoters to be combined through Gibson assembly. Added Prefix and Suffix through PCR with primers with overhangs.

Source

Genomic sequence from Saccharomyces cerevisiae CEN.PK2

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pTEF1	1,412	feature/promoter, promoter
pSUC2	413,541	feature/promoter, promoter

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1603001/1

Member of these Collections

• iGEM Parts Registry