BBa_K1603001

BBa_K1603001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1603001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Hellgren
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 05:57:31

pTEF1-pSUC2



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_K1603001_sequence (Version 1)

    Description

    The high expression pTEF1 promoter connected to pSUC2 promoter. Can be used for induced high expression of any coding part in Saccharomyces cerevisiae at low ATP levels.

    Notes

    Added 20 overlapping base pairs between the two gene fragments through PCR with primers with overhangs. This allowed the two promoters to be combined through Gibson assembly. Added Prefix and Suffix through PCR with primers with overhangs.

    Source

    Genomic sequence from Saccharomyces cerevisiae CEN.PK2

    Sequence Annotation Location Component / Role(s)
    pTEF1
    pSUC2
    		1,412
    413,541
    		feature/promoter promoter
    feature/promoter promoter

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1603001/1
     

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