Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Elisa Godino
Date created: 2015-08-17 11:00:00
Date modified: 2015-09-11 09:15:32
araC-pBAD + beta-carotene
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1604020_sequence (Version 1) |
Description
This Composite Biobrick is created by standard assembly of Part BBa_K731201 and Part BBa_K274200, which are submitted by previous iGEM teams. The whole cassette is on plasmid pSB1C3 (high copy, Chloramphenicol resistance).BBa_K274200 contains the enzymes that convert colourless farnesyl pyrophosphate to orange beta-carotene. The carotene operon contains CrtE, CrtB, CrtI and CrtY each one with individual ribosome binding site. This Composite Biobrick has been put under inducible araC-pBAD promoter that is positively regulated by arabinose.
Notes
Standard assembly between BBA_K731201 and a PCR amplification of BBa_K274200.BBa_K274200 was extracted by the 2012 distribution kit in pSB1A2 and it was amplified by PCR to restore a missing SpEI site.
| Access | Instance | Definition |
| public public | BBa_K731201 BBa_K274200 | BBa_K731201 BBa_K274200 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K731201 BBa_K274200 | 1,1203 1212,5766 | BBa_K731201 BBa_K274200 |