BBa_K1611000

BBa_K1611000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1611000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Frederic Ros

Date created: 2015-09-14 11:00:00

Date modified: 2015-12-17 01:03:34

IFNgamma

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1611000_sequence (Version 1)

Description

Murine IFN gamma cytokine codon optimized to give the best yield in yeast S. cerevisiae. It promotes T cell activation, upregulates MHC-I and is pro-inflammatory.

Notes

We performed a PCR site directed mutagenesis removing an enzyme restriction site in order to make it biobrick compatible. The mutation performed is silencious.

Source

This sequence was synthesised by IDT based on the genomic murine IFN gamma.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
c.198G>C	198,198	feature/s_mutation, point_mutation
stop	466,471	stop_codon, feature/stop
start	1,3	feature/start, start_codon
cds	1,468	CDS, feature/cds

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1611000/1

Member of these Collections

• //chassis/eukaryote/yeast
• iGEM Parts Registry