BBa_K1611000

BBa_K1611000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1611000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frederic Ros
Date created: 2015-09-14 11:00:00
Date modified: 2015-12-17 01:03:34

IFNgamma



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K1611000_sequence (Version 1)

    Description

    Murine IFN gamma cytokine codon optimized to give the best yield in yeast S. cerevisiae. It promotes T cell activation, upregulates MHC-I and is pro-inflammatory.

    Notes

    We performed a PCR site directed mutagenesis removing an enzyme restriction site in order to make it biobrick compatible. The mutation performed is silencious.

    Source

    This sequence was synthesised by IDT based on the genomic murine IFN gamma.

    Sequence Annotation Location Component / Role(s)
    c.198G>C
    stop
    start
    cds
    		198,198
    466,471
    1,3
    1,468
    		feature/s_mutation point_mutation
    stop_codon feature/stop
    feature/start start_codon
    CDS feature/cds

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1611000/1
     

    Attachments

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