| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1611001_sequence (Version 1)
|
Description
Murine IFN gamma cytokine codon optimized to give the best yield in yeast S. cerevisiae. It promotes T cell activation, upregulates MHC-I and is pro-inflammatory. MAT alpha is the peptide leader used as a secretion signal and fused to IFN gamma without ATG.
Notes
We performed a PCR site directed mutagenesis removing an enzyme restriction site in order to make it biobrick compatible. The mutation performed is silencious.
Source
MAT alpha was amplified from the S. cerevisiae genome. IFN gamma was synthesised by IDT based on the genomic murine IFN gamma. Both sequences have been assembled by golden gate to make a single fusion protein.
