Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frieda Anna Sorgenfrei
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-18 09:22:07
ATP Aptamer JAWS1 Spinach2
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_K1614014_sequence (Version 1) |
Description
Created from Part:BBa_K1614011 to sense ATP with a fluorescent read out.Notes
Cloned without restriction enzymes to avoid issues occurring from conserved cut sites. For more details see RFC 110.Source
Synthentic created with JAWS with Spinach II aptamer (Babendure, J.R., Adams, S.R., and Tsien, R.Y. (2003). Aptamers switch on fluorescence of triphenylmethane dyes. Journal of the American Chemical Society 125, 14716-14717.) and ATP aptamer| Sequence Annotation | Location | Component / Role(s) |
| G required for transcription T7 promotor Spinach II part1 optimized stem part1 ATP aptamer optimized stem part2 Spinach II part2 HDV | 30,30 13,29 31,65 66,73 74,130 131,138 139,176 177,261 | feature/misc sequence_feature feature/promoter promoter sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature |