Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frieda Anna Sorgenfrei
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-16 05:09:22
Hammerhead Ribozyme and Malchite Green Aptamer in BBF RFC 110 transcription cassette
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K1614016_sequence (Version 1) |
Description
Hammerhead Ribozyme (HHR) and Malchite Green (MG) Aptamer in BBF RFC 110 transcription cassette for live tracking of in vitro transcription.Notes
Cloned without restriction enzymes to avoid issues occurring from conserved cut sites. For more details see BBF RFC 110.Source
Synthetic, Malchit Green aptamer (Grate, D., and Wilson, C. (1999). Laser-mediated, site-specific inactivation of RNA transcripts. Proceedings of the National Academy of Sciences of the United States of America 96, 6131-6136.) HHR (Prody, G.A., Bakos, J.T., Buzayan, J.M., Schneider, I.R., and Bruening, G. (1986). Autolytic processing of dimeric plant virus satellite RNA. Science 231, 1577-1580)| Sequence Annotation | Location | Component / Role(s) |
| T7 promotor G required for transcription HHR Malachite Green Aptamer HDV | 13,29 30,30 31,84 85,122 123,206 | promoter feature/promoter feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc |