BBa_K1614025

BBa_K1614025 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1614025
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Heid
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 07:05:08

Ketamin-dependent self cleaving F8 - readout DNAzyme



    • Details

    Types
    DnaRegion

    Roles
    DNA

    sequence_feature

    Sequences BBa_K1614025_sequence (Version 1)

    Description

    Construct consisting of a ketamin-switched F8 DNAzyme linked to a stemmed HRP mimicking DNAzyme.

    If there no ketamine binds to aptamer the F8 is inactive and HRP mimicking DNAzyme is stemmed, so that no hemin could bind and there is no peroxidase activity.

    By binding of ketamin to the aptamer, F8 is activated and cleaves the stemming part, so the HRP mimicking DNAzyme is activated, peroxidase activity could be used for different readout techniques.

    Stems and Aptamer generated by MAWS and JAWS software.

    Notes

    Aptamer generated by MAWS software.
    Stems optimized by JAWS software.

    Source

    Synthetic.

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1614025/1
     

    Attachments

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