BBa_K1615058

BBa_K1615058 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1615058

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Brooke Rothschild-Mancinelli

Date created: 2015-09-01 11:00:00

Date modified: 2015-09-02 03:41:34

Heroin esterase fused to CBDcenA+Linker in RFC25 driven by LacI promoter

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1615058_sequence (Version 1)

Description

Heroin Esterase fused N terminally to CBDcenA + C terminal linker driven by the IPTG inducible PLlac 0-1 promoter.

Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.

Source

Heroin Esterase from Rhodococcus erythropolis. CBDcenA from Cellulomonas fimi. Lac expression cassette from iGEM10_Washington.

Components

Access	Instance	Definition
public	BBa_K314103	BBa_K314103
public	BBa_B0105	Scar 25
public	BBa_K1615048	BBa_K1615048

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K314103	1,1638	BBa_K314103
BBa_B0105	1639,1644	Scar 25
BBa_K1615048	1645,3036	BBa_K1615048

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1615058/1

Member of these Collections

• iGEM Parts Registry