BBa_K1615066

BBa_K1615066 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1615066

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Brooke Rothschild-Mancinelli

Date created: 2015-09-01 11:00:00

Date modified: 2015-09-02 04:02:33

Heroin Esterase fused to Linker-CBDcipA-linker driven by LacI promoter

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1615066_sequence (Version 1)

Description

Heroin Esterase fused N terminally to CBDcipA with N and C terminal linkers driven by the IPTG inducible PLlac 0-1 promoter.

Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.

Source

Heroin Esterase from Rhodococcus erythropolis. CBDcipA from Clostridium thermocellum. Lac expression cassette from iGEM10_Washington.

Components

Access	Instance	Definition
public	BBa_K314103	BBa_K314103
public	BBa_K1319106	Scar25 ATG
public	BBa_K1615056	BBa_K1615056

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K314103	1,1638	BBa_K314103
BBa_K1319106	1639,1653	Scar25 ATG
BBa_K1615056	1654,3339	BBa_K1615056

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1615066/1

Member of these Collections

• iGEM Parts Registry