BBa_K1615066

BBa_K1615066 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1615066
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brooke Rothschild-Mancinelli
Date created: 2015-09-01 11:00:00
Date modified: 2015-09-02 04:02:33

Heroin Esterase fused to Linker-CBDcipA-linker driven by LacI promoter



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1615066_sequence (Version 1)

Description

Heroin Esterase fused N terminally to CBDcipA with N and C terminal linkers driven by the IPTG inducible PLlac 0-1 promoter.

Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.

Source

Heroin Esterase from Rhodococcus erythropolis. CBDcipA from Clostridium thermocellum. Lac expression cassette from iGEM10_Washington.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1615066/1