BBa_K1616003

BBa_K1616003 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K1616003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Johanna Chesnel

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-18 03:39:05

VVD link to YC155 (YFP Cter split)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1616003_sequence (Version 1)

Description

Vivid (VVD) is the smallest known Light???oxygen???voltage (LOV) domain protein and photo-inducible dimer. Isolated from Neurospora crassa, VVD forms a homodimer in response to a blue-light stimulus.
Then, a split protein is a protein whose sequence has been divided into two (or more) different parts. The yellow-fluorescent (YFP) protein will only express fluorescence when its two parts will be reunited.
The part is coding for the homodimer VVD link by an integration of specific sequence to the C terminal of the YFP split.

Notes

The sequence of VVD had 2 illegal sites PstI; that have been removed.

Source

Assembly the sequence of photoreceptor VVD (without illegal site), a linker(1) and then the C terminal of YFP split(1)

(1) Tom Kerppola, Ph. D, investigator at the Howard Hughes Medical Institute as well as Professor in the University of Michigan

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
VVD	1,450	feature/protein, CDS
Linker	451,502	feature/protein, CDS
YC155	511,756	feature/protein, CDS

Other Properties

• igem#experience: None
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1616003/1

Member of these Collections

• iGEM Parts Registry