BBa_K1616004

BBa_K1616004 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K1616004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Johanna Chesnel

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-18 03:40:44

VVD linked to YN155 (YFP Nter split)

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1616004_sequence (Version 1)

Description

Vivid (VVD) is the smallest known Light???oxygen???voltage (LOV) domain protein and photo-inducible dimer. Isolated from Neurospora crassa, VVD forms a homodimer in response to a blue-light stimulus. Then, a split protein is a protein whose sequence has been divided into two (or more) different parts. The yellow-fluorescent (YFP) protein will only express fluorescence when its two parts will be reunited. The part is coding for the homodimer VVD link by an integration of specific sequence to the N terminal of the YFP split.

Notes

(1) Tom Kerppola, Ph. D, investigator at the Howard Hughes Medical Institute as well as Professor in the University of Michigan
Hu CD, Chinenov Y, Kerppola TK. Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol Cell. 2002;9(4):789???98.

Source

Assembly the sequence of photoreceptor VVD (without illegal site), a linker(1) and then the N terminal of YFP split(1)

Other Properties

• igem#experience: None
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1616004/1

Member of these Collections

• iGEM Parts Registry