End-rev

BBa_K1616006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1616006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Johanna Chesnel

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-17 11:02:11

Endolysin reversed

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1616006_sequence (Version 1)

Description

Holin and endolysin are toxins; after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bl??si, 1995)



This part is the sequence reverse of the Holin/ Endolysin; thus this part can be used with a reverse promoter.

Young, R., & Bl??si, U. (1995). Holins: Form and function in bacteriophage lysis. In FEMS Microbiology Reviews (Vol. 17, pp. 191???205). http://doi.org/10.1016/01686445(94)00079-4

Notes

The illegal sites have been checked

Source

This sequence is the reverse sequence of the holin/endolysin system.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Endolysin	1,514	CDS, feature/protein

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K1616006/1

Member of these Collections

• iGEM Parts Registry