Holin-rev

BBa_K1616007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1616007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Johanna Chesnel

Date created: 2015-09-16 11:00:00

Date modified: 2015-09-17 11:02:45

Holin reversed

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1616007_sequence (Version 1)

Description

Holin works with endolysin; they create a toxic system, after a period of late-gene expression, holin, an inner permeabilizing protein creates holes in the membrane in order to permeabilize it and then endolysin, a muralytic enzyme traverses the holes creating by holin and degraded the wall cell. This complex induce cell lysis of bacteria. (Young & Bl??si, 1995)

This part is the reversed sequence of the holin (BBa_K112805); thus this part can be used with a reversed endolysin (BBa_K1616006), reversed promoter, reversed RBS and reversed terminator.

Notes

The illegal sites have been checked

Source

This sequence is the reverses sequence of the BBa_K112805 (Holin).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Holin	1,676	CDS, feature/protein

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1616007/1

Member of these Collections

• iGEM Parts Registry