BBa_K1616013

BBa_K1616013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1616013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Johanna Chesnel

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-18 02:42:30

ccdB reversed - RBS and double T7 terminator

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1616013_sequence (Version 1)

Description

ccdB comes from the ccd system in Escherichia coli F plasmid and acts as a gyrase poison.
The CcdB protein, constitutively expressed by P1010, is lethal to most of the BioBrick cell strains, onlyDB3.1 is resistant.

Notes

Illegal sites have been checked.

Source

This part is composed of ccdB reversed, RBS reversed and the double terminator T7 reversed.

Components

Access	Instance	Definition
public	BBa_B0025	BBa_B0025
public	BBa_K1616012	ccdB-rev
public	BBa_K1616011	RBS-rev

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0025	1,129	BBa_B0025
BBa_K1616012	138,553	ccdB-rev
BBa_K1616011	562,575	RBS-rev

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1616013/1

Member of these Collections

• iGEM Parts Registry