Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Johanna Chesnel
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 06:53:36
pDawn system
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1616019_sequence (Version 1) |
Description
After reading many papers to select an appropriate light-sensing system to use for the kill switch, our team came across the pDawn and pDusk plasmids. Those plasmids employ bluelight photoreceptors to confer light-repressed or light-induced gene expression in with up to 460-fold induction upon illumination. Controlled by this system, we thought to add toxins in it to kill our bacteria whenever we want(Ohlendorf, Vidavski, Eldar, Moffat, & M??glich, 2012).They are not dependent on nonnative chromophores that are often supplied exogenously and do not need any introduction of cofactor-synthesis genes. Moreover, they are practical to use since current optogenetic tools require multiple plasmid components.
Finally, they deal with many limitations in a one-plasmid system which is based on the YF1/FixJ system.
Notes
The original sequence of pDawn have some illegal sites that have been removed.Source
The pDawn & pDusk system, have been optimized and constructed in the way that the toxins sequences we introduce in our construction are in fact the reverse sequences.| Sequence Annotation | Location | Component / Role(s) |
| lambda pR promoter cI-LVA FixK2 promoter YF1 YF1/FixJ bicistronic operon | 136,184 356,1102 1125,1372 1743,2876 2876,3493 | feature/promoter promoter CDS feature/cds feature/promoter promoter CDS feature/cds sequence_feature feature/misc |