Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Johann Bauerfeind
Date created: 2015-09-14 11:00:00
Date modified: 2015-09-23 06:07:18
Modular Flagellin - FliC_MCS
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1617000_sequence (Version 1) |
Description
= Contructing a multimodular Flagellin =1 Theory
1.1 Aim
Design of a functional flaggelin variant which does not contain the variable d3 flagellin domain anymore but instead carries a polylinker region (MCS). This polylinker region is the product of an inserted multi cloning side in place of the endogeneous d3 coding sequence.
Notes
= 1. Modularity of D3 Domain == 2. Substitution of Methionins =
= 3. Removing restriction sites =
= 4. Compatibility of Multi-Cloning-Site =
Source
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)Gene ID: 949101
Synonyms: ECK1922; flaF; H; hag; JW1908
| Sequence Annotation | Location | Component / Role(s) |
| Start ATG M144A BBa_K1617010 MCS Delete Xba1 site M401G Delete Pst1 site Delete BamHI site s_mutation Delete Pst1 site Delete Spe1 site Delete Pst1 site | 1,3 430,432 577,659 577,659 227,227 1203,1205 305,305 551,551 662,662 713,713 1016,1016 1290,1290 | feature/start start_codon sequence_feature feature/misc feature/BioBrick engineered_region sequence_feature feature/dna feature/s_mutation point_mutation sequence_feature feature/misc point_mutation feature/s_mutation point_mutation feature/s_mutation feature/s_mutation point_mutation feature/s_mutation point_mutation point_mutation feature/s_mutation point_mutation feature/s_mutation |