Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marc-Sven Roell, Caroline Skar Mj??nes, and Hanna Noordzij
Date created: 2015-08-31 11:00:00
Date modified: 2015-09-05 11:55:31
medh2 (NAD+ dependent methanol dehydrogenase)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1619001_sequence (Version 1) |
Description
Methanol dehydrogenase (medh) is an NAD-dependent dehydrogenase catalyzing the following reaction:CH3OH + NAD(+) → CH2O + NADH
Or in other words, catalyzes methanol to formaldehyde with the use of NAD(+).
For this enzyme to work in E. coli only this one gene is needed. The enzyme can use the E. coli's native NAD(+) resources to catalyze the reaction.
The medh gene is cloned from Bacillus methanolicus. Specifically, the mdh2 gene.
Mdh2 is chosen because of its efficiency in vivo: M??ller, J. E. N. et al. Engineering Escherichia coli for methanol conversion. Metab. Eng. 28, 190???201 (2015).
The gene is successfully expressed in E. coli.
Notes
noneSource
The gene is directly cloned from genomic DNA. Used sequence source for designing primers: http://www.genome.jp/dbget-bin/www_bget?bmet:BMMGA3_03335| Sequence Annotation | Location | Component / Role(s) |
| Stop codon Medh2 Start codon | 1156,1158 1,1156 1,3 | stop_codon feature/stop CDS feature/cds feature/start start_codon |