Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Celio Dias Santos Jr
Date created: 2015-09-02 11:00:00
Date modified: 2015-09-13 03:25:58
Promoter element Zasp
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K1620001_sequence (Version 1) |
Description
It is a zinc activity sensing promoter element. The regulation of this promoter is Zur dependant and positive in absent of zinc or other divalent metallic cations in medium. The Zur protein binds those cations repressing the expression of the gene downstream Zasp element. In our project, it was used to trigger the expression of killer genes downstream this element. Since ZnuABC activity is extremelly sensitive to low zinc concentrations, we believe that this component will be usefull for other teams in order to its inducible feature and precise functioning.Notes
We have identified two sequences of this promoter (one in sense strand and another in the antisense strand). In order to turn this sequence more feasible we used a hybrid final sequence. The final sequence was believed to promote for both sites.Source
The promoter element was designed through bioinformatic analysis of 500bp frame upstream genes of operon ZnuABC from E. coli K12 MG16555 genome. Then, it was synthesized by IDT through G-block and further assembled by 3A assembly method.| Sequence Annotation | Location | Component / Role(s) |
| Promoter Zasp | 1,50 | feature/promoter promoter |