Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Celio Dias Santos Jr
Date created: 2015-09-02 11:00:00
Date modified: 2015-09-13 03:51:31
small heat shock protein IbpA
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1620002_sequence (Version 1) |
Description
The IbpA is a small heat shock protein. It is expressed under stress conditions which associates with aggregated proteins. Together with IbpB, to stabilize and protect them from irreversible denaturation and extensive proteolysis during heat shock and oxidative stress. Aggregated proteins bound to the IbpAB complex are more efficiently refolded and reactivated by the ATP-dependent chaperone systems ClpB and DnaK/DnaJ/GrpE. Its activity is ATP-independent. It is extensively documented in the following link:Notes
This part was made using original codon frequencies of E. coli.Source
This part was designed from gene IbpA of E. coli K12 MG16555 genome, and synthesized by IDT. PCR amplifications were carried out using primers showed bellow and the 3A assembly method was made.PCR primers:
Fwd: 5' - GAA TTC GCG GCC GCT TCT AGA GAT GCG TAA CTT TGA TTT ATC - 3'
Rev: 5' - TAC TAG TAG CGG CCG CTG CAG TTA GTT GAT TTC GAT ACG GC - 3'