Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Celio Dias Santos Jr
Date created: 2015-09-02 11:00:00
Date modified: 2015-09-13 03:57:27
Zinc uptake regulation protein - Zur
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1620004_sequence (Version 1) |
Description
Acts like a negative controlling element of promoter Zasp (BBa_K1620001) by use of Zn(II) as a cofactor to bind the operator of the repressed genes (znuACB). In our project it was used to regulate a kill switch based on zinc concentration to promote cellular death.Notes
The designed gene was as provided by E. coli genome, with the same codon frequencies.Source
Designed from gene "zur" from E. coli K12 MG-1655 genome. Synthesized as G-block by IDT. PCR amplified using following primers:Fwd:
5' - GAA TTC GCG GCC GCT TCT AGA GAT GGA AAA GAC CAC AAC GCA - 3'
Rev:
5' - TAC TAG TAG CGG CCG CTG CAG TTA ACG CGG TTT CTT TTT CA - 3'
The assembly was made through 3A assembly method.