Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lara Stuehn, Ramona Emig, Julika Neumann, Rabea Jesser
Date created: 2015-08-27 11:00:00
Date modified: 2015-09-15 05:46:27
gag/tat/pol/env - polyepitopic antigen derived from HIV-1
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1621004_sequence (Version 1) |
Description
There are many epitopes known from the Human Immunodeficiency Virus 1, comprising envelope proteins as well as glycoproteins amongst others. For our purposes we found a paper introducing a multi-epitopic recombinant protein that is constructed with six different epitopes. The single epitopes are two of the HIV-1-trans-activating (tat) encoding region, one epitope of the reverse transcriptase, one of the p24 protein, one of the envelope protein gp41 and one of gp120.The HI Virus itself is highly immunogenic and underlies strict safety regulations. The great advantage of the multi-epitopic protein is that it does not have any immunogenic properties and is only built with small parts of functional proteins but still combines some of the most important epitopes present on the virus.
Notes
The sequence was codon optimized for expression in E.coli with the codon optimization tool from IDT.Source
The sequence for this part was obtained from Rahimi et al. (2015) and synthesized by Integrated DNA Technologies.| Sequence Annotation | Location | Component / Role(s) |
| HIV gag/tat/pol/env epitopes | 4,543 | CDS feature/cds |